Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors
作者： H. Akiyama , J.-E. Kim , K. Nakashima , G. Balmes , N. Iwai
关键词: Stem cell 、 Mesenchymal stem cell 、 Intramembranous ossification 、 Mesenchyme 、 Biology 、 Molecular biology 、 Chondrogenesis 、 Cellular differentiation 、 Limb bud 、 Cell fate determination
摘要: The transcription factor Sox9 is expressed in all chondroprogenitors and has an essential role chondrogenesis. also other tissues, including central nervous system, neural crest, intestine, pancreas, testis, endocardial cushions, plays a crucial cell proliferation differentiation several of these tissues. To determine the fate Sox9-expressing cells during mouse embryogenesis, we generated mice which Cre recombinase gene preceded by internal ribosome entry site was inserted into 3′ untranslated region (Sox9-Cre knock-in). In developing skeleton, before Runx2, early osteoblast marker gene. Cell mapping using Sox9-Cre;ROSA26 reporter (R26R) revealed that limb bud mesenchymal gave rise to both chondrocytes osteoblasts. Furthermore, mutant Osterix inactivated exhibited lack endochondral intramembranous ossification mature osteoblasts comparable with Osterix-null mutants. addition, contributed tendon synovium formation. By Sox9-Cre;R26R mice, were able systematically follow from embryonic day 8.0 17.0. Our results showed formation types spinal cord, epithelium mesenchyme testis. Thus, our strongly suggest osteo-chondroprogenitor cells, as well progenitors variety are derived precursors embryogenesis.
harvard.edu
unirioja.es
jstor.org 
sci-hub.se 参考文章(28)
Andrew Elefanty, Andrew Elefanty, Christine Biben, Richard P Harvey, Richard P Harvey, Edouard G Stanley, Louise Barnett, Louise Barnett, Frank Koentgen, Lorraine Robb, Efficient Cre-mediated deletion in cardiac progenitor cells conferred by a 3'UTR-ires-Cre allele of the homeobox gene Nkx2-5. The International Journal of Developmental Biology. ,vol. 46, pp. 431- 439 ,(2002) , 10.1387/IJDB.12141429
Qi Zhao, Heidi Eberspaecher, Véronique Lefebvre, Benoit de Crombrugghe, Parallel expression of Sox9 and Col2a1 in cells undergoing chondrogenesis. Developmental Dynamics. ,vol. 209, pp. 377- 386 ,(1997) , 10.1002/(SICI)1097-0177(199708)209:4<377::AID-AJA5>3.0.CO;2-F
Weimin Bi, Jian Min Deng, Zhaoping Zhang, Richard R. Behringer, Benoit de Crombrugghe, Sox9 is required for cartilage formation. Nature Genetics. ,vol. 22, pp. 85- 89 ,(1999) , 10.1038/8792
Philippe Soriano, Generalized lacZ expression with the ROSA26 Cre reporter strain Nature Genetics. ,vol. 21, pp. 70- 71 ,(1999) , 10.1038/5007
Malcolm Logan, James F. Martin, Andras Nagy, Corrinne Lobe, Eric N. Olson, Clifford J. Tabin, Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer. Genesis. ,vol. 33, pp. 77- 80 ,(2002) , 10.1002/GENE.10092
Jamie W. Foster, Marina A. Dominguez-Steglich, Silvana Guioli, Cheni Kwok, Polly A. Weller, Milena Stevanović, Jean Weissenbach, Sahar Mansour, Ian D. Young, Peter N. Goodfellow, J. David Brook, Alan J. Schafer, Campomelic dysplasia and autosomal sex reversal caused by mutations in an SRY-related gene. Nature. ,vol. 372, pp. 525- 530 ,(1994) , 10.1038/372525A0
Marie-Christine Chaboissier, Akio Kobayashi, Valerie IP Vidal, Susanne Lützkendorf, Henk JG van de Kant, Michael Wegner, Dirk G de Rooij, Richard R Behringer, Andreas Schedl, Functional analysis of Sox8 and Sox9 during sex determination in the mouse. Development. ,vol. 131, pp. 1891- 1901 ,(2004) , 10.1242/DEV.01087
Louis-Bruno Ruest, Marcus Dager, Hiromi Yanagisawa, Jeroen Charité, Robert E Hammer, Eric N Olson, Masashi Yanagisawa, David E Clouthier, dHAND-Cre transgenic mice reveal specific potential functions of dHAND during craniofacial development. Developmental Biology. ,vol. 257, pp. 263- 277 ,(2003) , 10.1016/S0012-1606(03)00068-X
Patricia Ducy, Rui Zhang, Valérie Geoffroy, Amy L Ridall, Gérard Karsenty, Osf2/Cbfa1: A Transcriptional Activator of Osteoblast Differentiation Cell. ,vol. 89, pp. 747- 754 ,(1997) , 10.1016/S0092-8674(00)80257-3
T Komori, H Yagi, S Nomura, A Yamaguchi, K Sasaki, K Deguchi, Y Shimizu, R.T Bronson, Y.-H Gao, M Inada, M Sato, R Okamoto, Y Kitamura, S Yoshiki, T Kishimoto, Targeted Disruption of Cbfa1 Results in a Complete Lack of Bone Formation owing to Maturational Arrest of Osteoblasts Cell. ,vol. 89, pp. 755- 764 ,(1997) , 10.1016/S0092-8674(00)80258-5