The polymerase chain reaction for Mycoplasma gallisepticum detection.

摘要: Summary On the basis of aligned 16S rRNA sequences Mollicutes, a pair primers was chosen for detection Mycoplasma gallisepticum. When used in polymerase chain reaction (PCR), detected specific amplification all Mg strains tested, yielding an expected 330 bp product. Amplification not when other Mollicutes or E. coli were as PCR templates. SPF chickens experimentally inoculated with two M. gallisepticum iowae. Tracheal swabs collected 8, 15, 20 and 28 days after inoculation, cultured mycoplasma tested by PCR. products hybridization digoxigenin‐labeled probe chemilumines‐cence. The results showed that culture positive 49/73 while 70/72 samples. Thus, can provide sensitive, specific, rapid non‐radio‐active method detecting