PCR slippage across the ML-2 microsatellite of the Cryptosporidium MIC1 locus enables development of a PCR assay capable of distinguishing the zoonotic Cryptosporidium parvum from other human infectious Cryptosporidium species.

摘要: Summary Cryptosporidium are ubiquitous and significant enteropathogens of all classes vertebrates a major cause human morbidity mortality worldwide. Of the 24 recognized species, zoonotic Cryptosporidium parvum host-specific hominis majority cases cryptosporidiosis. Here, we report on structural transcriptional variability between C. parvum C. hominis at MIC1 locus, which encodes microneme localized thrombospondin-like domain containing protein previously demonstrated to be critical for host cell infection by C. parvum. We demonstrate, using reverse transcription quantitative PCR with aid genomic data from EuPathDB site, that transcribed product in is both truncated significantly down-regulated sporozoite. hypothesize CpMIC1 may genetic factor involved facilitating wider range comparison specific C. hominis. Furthermore, show presence microsatellite (ML-2) within MIC-1 locus enables development marker can rapidly distinguish other infectious species due reproducible slippage across ML-2 microsatellite. Additionally, demonstrate this tightly linked GP60 commonly used characterization isolates. This should provide robust additional tool rapid identification species.