Zoonotic Cryptosporidium Species in Animals Inhabiting Sydney Water Catchments.

摘要: Cryptosporidium is one of the most common zoonotic waterborne parasitic diseases worldwide and represents a major public health concern water utilities in developed nations. As animals catchments can shed human-infectious oocysts, determining potential role dissemination to drinking sources crucial. In present study, total 952 animal faecal samples from four dominant species (kangaroos, rabbits, cattle sheep) inhabiting Sydney’s were screened for presence using quantitative PCR (qPCR) positives sequenced at multiple loci. detected 3.6% (21/576) kangaroos, 7.0% (10/142) cattle, 2.3% (3/128) sheep 13.2% (14/106) rabbit screened. Sequence analysis region 18S rRNA locus identified C. macropodum hominis 4 17 isolates kangaroos respectively, parvum 6 respectively each ubiquitum 3 cuniculus 14 rabbits. All with exception macropodum. Subtyping 5’ half gp60 IbA10G2 (n = 12) IdA15G1 2) kangaroo samples; 4) IIaA18G3R1 samples, subtype XIIa 1) VbA23 9) Additional subset primers targeting conserved regions MIC1 gene 3’ end suggests that these represent substantial variants failed amplify as expected. The significance this finding requires further investigation but might be reflective ability variant infect animals. may have important implications management minimize risk health.