Suppression of phospholipase Dα1 induces freezing tolerance in Arabidopsis: Response of cold-responsive genes and osmolyte accumulation
作者： C.B. Rajashekar , Han-E Zhou , Yuwen Zhang , Weiqi Li , Xuemin Wang
DOI: 10.1016/J.JPLPH.2005.08.006
关键词: Gene silencing 、 Arabidopsis 、 Phospholipase 、 Phospholipase D 、 Cell biology 、 Biology 、 Osmolyte 、 Regulation of gene expression 、 Biochemistry 、 Lysophosphatidylethanolamine 、 Cold acclimation
摘要: Phospholipase D (PLD; EC 3.1.4.4) plays an important role in membrane lipid hydrolysis and mediation of plant responses to a wide range stresses. PLDalpha1 abrogation through antisense suppression Arabidopsis thaliana resulted significant increase freezing tolerance both non-acclimated cold-acclimated plants. Although PLDalpha1-deficient plants did not show the activation cold-responsive C-repeat/dehydration-responsive element binding factors (CBFs) their target genes (COR47 COR78), they accumulate osmolytes much higher levels than wild-type However, stronger expression COR47 COR78 response cold acclimation especially was observed Furthermore, slower CBF1 these compared Typically, accumulation Inhibition PLD activity by using lysophosphatidylethanolamine (LPE) also increased Arabidopsis, albeit lesser extent suppression. Exogenous LPE induced COR15a absence stimulus. These results suggest that key modulating osmolytes.
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