Filamentous Fungi as Expression Systems for Heterologous Proteins

摘要: A wide variety of heterologous genes have been introduced into filamentous fungi with the purpose producing encoded protein, usually in a secreted form. There are several reasons for electing to use as expression hosts and principal amongst them that many species naturally secrete native proteins (mainly hydrolytic enzymes facilitate their saprophytic lifestyle) apparent efficiency, there is extensive commercial experience large-scale fungal cultivation extremely high yields some achieved. also success stories few providing hope other will be produced same facility equally yields. In main, such hopes not realised it this chapter discuss pitfalls present summary recent advances science underlying production from hoped lead rational improvement hosts. Our focus on because topic has reviewed previously (Archer Peberdy 1997; Gouka et al. 1997a; Radzio Kuck Maras 1999; Archer 2000; Conesa 2001a; 2001; Punt 2002) we cite these reviews where they summarise key issues provide useful reference original literature. We specifically yeast systems although (e.g. Saccharomyces cerevisiae, Kluyveromyces lactis, Pichia pastoris) successfully used at laboratory scales. Even S. cerevisiae which normally noted its capacity proteins, developed system commercially acceptable insulin human serum albumin. Thus, feasible modify an organism through combination mutagenesis targeted gene manipulation order achieve goal. advantage over all being by far best studied furnished best-developed genetics underpinned complete genome sequence increasing information structure function. can, therefore, learn apply knowledge appropriate provided recognise, seen below clear differences. The challenge explore develop hyphal become better producers proteins. To aim improved tools transformation, deletion), basic mechanisms protein secretion data species. emphasis throughout molecular basis manipulation.