Induction of cellular senescence by oncogenic RAS.
作者： Romi Gupta , Narendra Wajapeyee
DOI: 10.1007/978-1-62703-556-9_10
关键词: Cell biology 、 Neoplastic transformation 、 Senescence 、 Oncogene 、 Cancer cell 、 Fibroblast 、 Cell cycle checkpoint 、 Gene 、 Biology 、 Anti-apoptotic Ras signalling cascade
摘要: Cellular senescence is a state of permanent cell cycle arrest, which has been shown to prevent neoplastic transformation. Oncogenes are pro-proliferative genes that promote survival and proliferation cancer cells. Paradoxically, introduction an activated oncogene leads activation similar cellular senescence, referred as oncogene-induced senescence. Studies have function barrier Here, we describe protocol for inducing by oncogenic RAS in primary human fibroblast. The approach here general utility can be applied study induction other defined genetic elements, example, oncogenes.
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