Pioglitazone Improves Mitochondrial Function in the Remnant Kidney and Protects against Renal Fibrosis in 5/6 Nephrectomized Rats.

摘要: Pioglitazone is a type of peroxisome proliferator-activated receptor γ (PPARγ) agonist and has been demonstrated to be effective in chronic kidney diseases (CKD) treatment. However, the underlying mechanism involved renoprotection pioglitazone not fully revealed. In present study, renoprotective was investigated 5/6 nephrectomized (Nx) rats TGF-β1-exposed HK-2 cells. attenuated renal injury improved function, as examined by 24 h urinary protein, blood urea nitrogen plasma creatinine Nx rats. Renal fibrosis enhanced expressions profibrotic proteins TGF-β1, fibronectin collagen I caused were significantly alleviated pioglitazone. addition, protected mitochondrial functions stabilizing membrane potential, inhibiting ROS generation, maintaining ATP production activities complexes III, preventing cytochrome C leakage from mitochondria. also upregulated expression levels synthase β, COX NDUFB8, which downregulated Furthermore, increased fusion Opa-1 Mfn2 decreased fission protein Drp1 expression. The results imply that may exert effects through modulating electron transport chain dynamics CKD. Finally, these recoveries completely or partly inhibited GW9662, suggests at least PPARγ dependent. This study provides evidence for pharmacological treatment