Immunohistochemistry of Caspase3/CPP32 in human stomach and its correlation with cell proliferation and apoptosis

摘要: Caspase3/CPP32 is a member of the interleukin-1 beta-converting enzyme (ICE) or cell death effector (CED)-3 family, which involved in induction apoptosis and has been considered to be correlated with because most downstream their inducing pathway. We immunolocalized both normal neoplastic human gastric mucosa. then findings proliferation studied by Ki67 immunostaining apoptosis, was tested for DNA fragmentation situ using TdT-mediated dUTP biotin nick end labeling (TUNEL) method order examine possible biological significance turnover pathological tissues. immunoreactivity detected cytoplasm nuclei glandular epithelial cells, predominantly positive proliferative zone TUNEL foveolar epithelium non-neoplastic mucosa (n = 10) tumor cells adenoma 17) carcinoma 33). determined index (LI) Ki67, evaluating number same areas serial tissue sections computer-assisted image analysis. LI adenocarcinoma (78.6 +/- 12.6%) significantly [p < 0.0001] higher than that (43.8 8.9%) (24.2 9.0%). (17.1 10.3%) lower (33.1 19.8%) (42.4 15.8%). (1.9 2.1%) (6.0 3.5%), but not different from (3.0 2.9%). These results indicate associated an inhibition augmentation activity compared There tendency correlation between inverse LI, when all specimens, although did reach statistical significance. also suggest development regulation apoptotic stomach.