Trans-activation by HIV-1 Tat via a heterologous RNA binding protein

作者: Mark J. Selby , B.Matija Peterlin

DOI: 10.1016/0092-8674(90)90121-T

关键词: Binding proteinPeptide sequenceBiologyTrans-ActivatorsMolecular biologyTranscription (biology)RNA-binding proteinRNADNA-binding proteinCapsid

摘要: The HIV-1 trans-activator Tat increases levels of viral gene expression and replication. target for is an RNA stem-loop called TAR, located at the 5' end all transcripts. To study mechanism action map functional domains Tat, we fused to coat protein bacteriophage MS2, binding protein. TAR in LTR was replaced by operator, hybrid Tat-coat trans-activated LTRs containing either or operator sequences. Mutations that weaken vitro led decreased trans-activation vivo. Deletions within identified activation Tat. These experiments suggest can occur independently DNA proteins exerts its effects on transcription directly interacting with stem-loop.

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