The Isolation of Novel Phage Display-Derived Human Recombinant Antibodies Against CCR5, the Major Co-Receptor of HIV

摘要: Abstract Selecting for antibodies against specific cell-surface proteins is a difficult task due to many unrelated that are expressed on the cell surface. Here, we describe method screen antibody-presenting phage libraries native proteins. We applied this isolate selectively recognize CCR5, which major co-receptor HIV entry (consequently, playing pivotal role in transmission and pathogenesis). employed screening strategy by using cells co-express GFP along with an excess of control do not express these (and otherwise identical CCR5-expressing cells). These intended remove most phages bind nonspecifically; thus leading enrichment presenting anti-CCR5-specific antibodies. Subsequently, CCR5-presenting were quantitatively sorted flow cytometry, bound eluted, amplified, used for...