Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor.
作者： Tajib Mirzabekov , Norbert Bannert , Michael Farzan , Wolfgang Hofmann , Peter Kolchinsky
关键词:
摘要: Seven-transmembrane segment, G protein-coupled receptors (GPCRs) play important roles in many biological processes which pharmaceutical intervention may be useful. High level expression and native purification of GPCRs are steps the biochemical structural characterization these molecules. Here, we describe enhanced mammalian cell a codon-optimized variant chemokine receptor CCR5, GPCR that plays central role entry human immunodeficiency virus-1 (HIV-1) into immune cells. CCR5 could solubilized its state as determined by ability to precipitated 2D7, conformation-dependent anti-CCR5 antibody, HIV-1 gp120 envelope glycoprotein. The 2D7 antibody recognized immature mature forms equally, whereas preferentially form, result underscores for posttranslational modification coreceptor function. methods described herein contribute analysis likely applicable other GPCRs.
sci-hub.se 参考文章(38)
H G Khorana, Rhodopsin, photoreceptor of the rod cell. An emerging pattern for structure and function. Journal of Biological Chemistry. ,vol. 267, pp. 1- 4 ,(1992) , 10.1016/S0021-9258(18)48444-X
Richard Wyatt, Peter D. Kwong, Elizabeth Desjardins, Raymond W. Sweet, James Robinson, Wayne A. Hendrickson, Joseph G. Sodroski, The antigenic structure of the HIV gp120 envelope glycoprotein Nature. ,vol. 393, pp. 705- 711 ,(1998) , 10.1038/31514
Hyeryun Choe, Michael Farzan, Ying Sun, Nancy Sullivan, Barrett Rollins, Paul D Ponath, Lijun Wu, Charles R Mackay, Gregory LaRosa, Walter Newman, Norma Gerard, Craig Gerard, Joseph Sodroski, The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Primary HIV-1 Isolates Cell. ,vol. 85, pp. 1135- 1148 ,(1996) , 10.1016/S0092-8674(00)81313-6
Alexandra Trkola, Tatjana Dragic, James Arthos, James M. Binley, William C. Olson, Graham P. Allaway, Cecilia Cheng-Mayer, James Robinson, Paul J. Maddon, John P. Moore, CD4-dependent, antibody-sensitive interactions between HIV-1 and its co-receptor CCR-5. Nature. ,vol. 384, pp. 184- 187 ,(1996) , 10.1038/384184A0
K. Tan, J.-h. Liu, J.-h. Wang, S. Shen, M. Lu, Atomic structure of a thermostable subdomain of HIV-1 gp41. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 12303- 12308 ,(1997) , 10.1073/PNAS.94.23.12303
David C. Chan, Deborah Fass, James M. Berger, Peter S. Kim, Core Structure of gp41 from the HIV Envelope Glycoprotein Cell. ,vol. 89, pp. 263- 273 ,(1997) , 10.1016/S0092-8674(00)80205-6
Per A. Bullough, Frederick M. Hughson, John J. Skehel, Don C. Wiley, Structure of influenza haemagglutinin at the pH of membrane fusion Nature. ,vol. 371, pp. 37- 43 ,(1994) , 10.1038/371037A0
HongKui Deng, Rong Liu, Wilfried Ellmeier, Sunny Choe, Derya Unutmaz, Michael Burkhart, Paola Di. Marzio, Shoshana Marmon, Richard E. Sutton, C. Mark Hill, Craig B. Davis, Stephen C. Peiper, Thomas J. Schall, Dan R. Littman, Nathaniel R. Landau, Identification of a major co-receptor for primary isolates of HIV-1 Nature. ,vol. 381, pp. 661- 666 ,(1996) , 10.1038/381661A0
Tatjana Dragic, Virginia Litwin, Graham P. Allaway, Scott R. Martin, Yaoxing Huang, Kirsten A. Nagashima, Charmagne Cayanan, Paul J. Maddon, Richard A. Koup, John P. Moore, William A. Paxton, HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. ,vol. 381, pp. 667- 673 ,(1996) , 10.1038/381667A0
Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled Receptor Science. ,vol. 272, pp. 872- 877 ,(1996) , 10.1126/SCIENCE.272.5263.872