Loss of nuclear BRCA1 protein staining in normal tissue cells derived from BRCA1 and BRCA2 mutation carriers.
作者： Sylvia De Brakeleer , Marika Bogdani , Jacques De Grève , Julie Decock , Erica Sermijn
DOI: 10.1016/J.MRFMMM.2007.02.031
关键词: Immunocytochemistry 、 Breast cancer 、 Molecular biology 、 Biology 、 Genome instability 、 Staining 、 Cancer research 、 Mutation 、 Cancer 、 In vitro 、 Brca1 protein
摘要: Enhanced genomic instability has been recently reported in normal cells derived from BRCA1/2 mutation carriers when placed vitro non-physiological stress conditions. We present here original data which help to explain the observed instability. Leucocytes carriers, sporadic breast cancer patients and controls were prepared for BRCA1 immunocytochemistry. show that containing nuclear dot like structures are detectable about 80% of leucocytes patients, but absent majority as well BRCA2 (also their cells). Our results thus indicate is associated with a down-regulation protein accumulation structures. These suggest addition immunocytochemical or alternative molecular screening strategies might identify women high risk (ovarian) even underlying genetic defect remains undetectable.
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