A subanesthetic dose of isoflurane during postconditioning ameliorates zymosan-induced neutrophil inflammation lung injury and mortality in mice.

摘要: Anesthetic isoflurane (ISO) has immunomodulatory effects. In the present study, we investigated whether a subanesthetic dose of ISO (0.7%) protected against zymosan (ZY) induced inflammatory responses in murine lung and isolated neutrophils. At 1 6 hrs after ZY administration intraperitoneally, was inhaled for 1 hr, 24 hrs later, inflammation injury were assessed. We found that improved survival rate mice mitigated as characterized by histopathology, wet-to-dry weight ratio, protein leakage, function index. significantly attenuated ZY-induced neutrophil recruitment inflammation. This suggested downregulation (a) endothelial adhesion molecule expression myeloperoxidase (MPO) activity tissue polymorphonuclear neutrophils (b) chemokines, (c) proinflammatory cytokines BALF. Furthermore, nuclear translocation DNA-binding NF-κB p65 also reduced ISO. treatment inhibited iNOS activity, well subsequent nitric oxide generation. Consistent with these vivo observations, vitro studies confirmed blocked activation primary mouse challenged ZY. These results provide evidence 0.7% ameliorates ZY-treated