Mass transfer studies of Geobacter sulfurreducens biofilms on rotating disk electrodes

摘要: Electrochemical impedance spectroscopy has received significant attention recently as a method to measure electrochemical parameters of Geobacter sulfurreducens biofilms. Here, we use demonstrate the effect mass transfer processes on electron by G. biofilms grown in situ an electrode that was subsequently rotated. By rotating up 530 rpm, could control microscale gradients formed inside A 24% increase above baseline 82 µA be achieved with rotation rate 530 rpm. comparison, observed 340% using soluble redox mediator (ferrocyanide) limited transfer. Control also used quantify change biofilm during transition from turnover non-turnover. We found only one element impedance, interfacial resistance, changed significantly 900 4,200 Ω under and non-turnover conditions, respectively. ascribed this resistance overcome metabolism estimate value 3,300 Ω. Additionally, non-turnover, developed pseudocapacitive behavior indicative bound mediators. Pseudocapacitance estimated at 740 µF unresponsive electrode. The indicators acetate limitations Biotechnol. Bioeng. 2014;111: 285–294. © 2013 Wiley Periodicals, Inc.