Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture.

摘要: Mammalian circadian clock genes Per1 and Per2 are rhythmically expressed not only in the suprachiasmatic nucleus where mammalian exists, but also other brain regions peripheral tissues. The induced oscillation of Per after treatment with high concentrations serum or various drugs cultured cells suggests ubiquitous existence oscillatory mechanism. These treatments result a rapid surge expression Per1. It has been shown that multiple signaling pathways involved gene induction culture cells. We used dispersed primary cell made up mouse cerebellar granule to examine stimuli inducing mPer their neuronal tissues expressing genes. demonstrated mPer1, mPer2, mRNA was dependent on depolarization state controlled by extracellular KCl concentration culture. Nifedipine reduced mPer1 induction, suggesting depends intracellular calcium regulated through voltage-dependent Ca2+ channel. Transient observed elevating medium from 5 mM 25 mM. This increased suppressed calmodulin antagonist, CaMKII/IV inhibitor, MEK inhibitors. Addition pituitary adenylate cyclase-activating polypeptide-38 transient expression. mimicked dibutyryl-cAMP protein kinase A (PKA) results suggest Ca2+/calmodulin-dependent II/IV- PKA-dependent high-KCl PACAP-induced respectively, neural use for similar