poly)Phosphoinositide phosphorylation is a marker for plasma membrane in Friend erythroleukaemic cells

作者: André J. Rawyler , Ben Roelofsen , Karel W.A. Wirtz , Jos A.F. Op den Kamp

DOI: 10.1016/0014-5793(82)81260-X

关键词: Membrane lipidsKinasePlasmaBiochemistryMarker enzymesDistribution (pharmacology)MembraneCell fractionationPhosphorylationBiology

摘要: Upon subcellular fractionation of (murine) Friend erythroleukaemic cells (FELCs), purified plasma membranes were identified by their high enrichment in specific marker enzymes and typical membrane lipids. When FELCs incubated for short periods with 32Pi before cell fractionation, the lipid-bound radioactivity was almost exclusively present phosphatidylinositol-4-phosphate (DPI) phosphatidylinositol-4,5-bisphosphate (TPI), its distribution closely matched that markers. In addition, actively incorporated 32P from [gamma-32P]ATP into polyphosphoinositides, activities involved kinases again mostly enriched fraction.

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