Modulation of blood cell activation by four commonly used anticoagulants

摘要: In the past years, our group has made several observations suggesting that blood cells behave differently and when stimulated, release different levels of cytokines, depending on which anticoagulant been drawn into. The aim this study was therefore to compare effect four anticoagulants EDTA, citrate, heparin hirudin monocyte, neutrophil (PMN), platelet function in human whole blood. Human employed as an ex vivo model cytokine production protein secretion, lipopolysaccharide (LPS) induced tissue factor (TF) activity monocytes LPS tumor necrosis alpha (TNF alpha) were chosen parameters monocyte activation. Platelet 4 (PF4) secretion lactoferrin PMN activation, respectively. When stimulated with 5 ng/ml for 2 h, TF isolated from EDTA found be 2.9 mU/10(6) cells, whereas citrated, heparinized hirudinized 14.7, 24.7 28.5 TNF concentrations poor plasma (PPP) h increased 200, 400 350% respectively, compared Next, measured. PPP incubated 90 min contained 1170 (EDTA blood), 2880 (citrated 4220 (heparinized 5520 (hirudinized blood). studying parameter PF4, without any stimuli 60 min, we 1180 while 469 ng/ml, citrate 440 217 Finally, low molecular weight compound Fragmin had no enhancing PF4 It is concluded used vitro experiments a large influence