Monocyte adherence results in selective induction of novel genes sharing homology with mediators of inflammation and tissue repair.

摘要: Adherence of monocytes to endothelial cells or extracellular matrices is likely play a critical role in triggering monocyte activation extravascular sites infection, chronic inflammatory disorders, tissue damage and neoplastic growth. We have constructed cDNA library from adhered for 30 min on plastic screened it by differential hybridization mRNA rapidly induced adherence. Two the isolated been identified as IL-1 beta superoxide dismutase. Sequence data three other adherence specific clones demonstrates presence ATTTA instability sequences their 3' untranslated regions signifying inflammation-associated genes. The deduced amino acid indicate open reading frames with sequence homologies family host defense cytokines, one them being IL-8. Of 14 initially identified, 4 analyzed induction expression. Although 3 were equally PMA LPS under nonadherent conditions, all showed distinct patterns matrix components cells. that we code unique gene products. These belong early cytokines involved inflammation cell growth, but which are differentially regulated different surfaces.