Targeting tail-anchored proteins into plant organelles.

摘要: The extensive evolution of intracellular compartmentalization requires highly selective mechanisms for protein targeting to distinct membrane systems. One the fundamental processes in is insertion proteins into biological membranes. efficient and accurate an important step their proper function different organelles, any error may lead mislocalization these with detrimental cellular effects. Posttranslational required a class tail-anchored (TA) proteins, which are characterized by transmembrane domain (TMD) near C terminus, correct destined (1). In PNAS, Xing et al. uncover pathway TA endoplasmic reticulum (ER) model plant Arabidopsis thaliana , plays unexpected role root hair growth (2). authors identify several key components guided entry (GET) complex that has conserved regulating insertion. However, contrast yeast animals, core GET system involves GET3 clade, suggesting ancient paralogs plants divergently as plant-specific organelle chaperones. The most extensively studied shuttling organelles mammalian cells. Structural biochemical investigations have provided insights molecular mechanism pathway, newly synthesized initially recognized cytosolic pretargeting (PTC), comprising SGT2 GET4–GET5, then transferred ATPase GET3. loaded will bind its receptor: ER-localized GET1–GET2 complex, subsequently promotes ER (Fig. 1 A ). recognition mediated … [↵][1]1To whom correspondence should be addressed. Email: ljiang{at}cuhk.edu.hk. [1]: #xref-corresp-1-1