Preparation of poly(L-lactic acid) nanofiber scaffolds with a rough surface by phase inversion using supercritical carbon dioxide.

摘要: Phase inversion using supercritical carbon dioxide (SC-CO2) has been widely used in the development of tissue engineering scaffolds, and particular attention given to obtaining desired morphology without additional post-treatments. However, main challenge this technique is difficulty generating a three-dimensional (3D) nanofiber structure with rough surface one step. Here, poly(L-lactic acid) (PLLA) 3D scaffold obtained via phase SC-CO2 by carefully choosing fabrication conditions porogens. It found that method can effectively modulate morphology, promote crystallization process semicrystalline polymer, induce formation structures on nanofibers. Meanwhile, porogen ammonium bicarbonate (AB) produce large pores, menthol improve interconnectivity between micropores A significant increase fiber diameter observed as content increases. Furthermore, may affect mutual transition α' α crystals PLLA during separation process. In addition, results protein adsorption, cell adhesion, proliferation assays indicate cells tend have higher viability scaffold. This combines characteristic properties solubility tailor polymeric which potential applications engineering.