The role of soluble proteins in generating aqueous outflow resistance in the bovine and human eye

摘要: Abstract Previous research has shown that wash-out in bovine and primate eyes can be greatly reduced by perfusing with buffer containing 5–15% serum. It was suggested protein diffusion from the iris root might raise vivo concentration trabecular meshwork to a level much higher than anterior chamber. In this study, we investigated effluent outflow pathways human eyes, its possible relationship wash-out, whether reduction of caused bulk effect. Bovine were placed under silicone oil perfused buffer. Outflow facility continuously determined while periodically collected surface eye, soluble determined. Separate studies conducted either albumin or γ-globulin through eyes. Theoretical models developed study transport into perfusion fluid. initial approximately 1% serum, lower 10–15% serum required prevent wash-out. Furthermore, rate change showed different dependence on volume did concentration. Human perfusate, decayed over longer time period. A statistically significant correlation existed between resistance both However, modelling due flow setting flowrate which then determines effluent. experiments indicated decreased not alone. These results suggest observed previous when proteins monkey general but may instead interactions particular protein(s).