Interaction between La3+ and MP-11 in the physiological solution
作者： Shaofen Guo , Qing Zhou , Tianhong Lu , Xiaolan Ding , Xiaohua Huang
DOI: 10.1016/J.ELECTACTA.2006.08.023
关键词: Electrocatalyst 、 Active center 、 Electrochemistry 、 Analytical chemistry 、 Circular dichroism 、 Random coil 、 Crystallography 、 Hemeprotein 、 Porphyrin 、 Chemistry 、 Heme 、 General Chemical Engineering
摘要: Abstract In this paper, the interaction between La 3+ and microperoxidase-11 (MP-11) in imitated physiological solution was investigated with electrochemical method, circular dichroism (CD) ultraviolet–visible (UV–vis) absorption spectroscopy. It found that ways MP-11 are different increasing molar ratio of MP-11. When is less than 2, mainly interacts metacetonic acid group heme molecules, causing increase non-planarity porphyrin cycle decrease content random coil conformation These structural changes would exposure extent active center thus, can promote reaction its electrocatalytic activity for reduction H 2 O at glassy carbon (GC) electrode. However, when larger 3, except binding to carbonyl oxygen group, also oxygen-containing groups amides polypeptide chains leading contents peptide molecule, comparing 2. similar Perhaps, molecule partially shield not favorable Thus, reversibility lower
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