Modeling Spontaneous Bone Metastasis Formation of Solid Human Tumor Xenografts in Mice.

摘要: The majority of cancer-related deaths are due to hematogenous metastases, and the bone marrow (BM) represents one most frequent metastatic sites. To study BM metastasis formation in vivo, efficient approach is based on intracardiac injection human tumor cells into immunodeficient mice. However, such a procedure circumvents early steps cascade. Here we describe development xenograft mouse models (balb/c rag2-/- severe combined (SCID)), which metastases spontaneously derived from subcutaneous (s.c.) primary tumors (PTs). As verified by histology, described methodology including ex vivo bioluminescence imaging (BLI) even enabled detection micrometastases BM. Furthermore, established sublines (PTs) corresponding using LAN-1 neuroblastoma xenografts as first example. In vitro “metastasis” assays (viability, proliferation, transmigration, invasion, colony formation) partially indicated pro-metastatic features LAN-1-BM compared LAN-1-PT subline. Unexpectedly, after s.c. re-injection mice, developed spontaneous less frequently than xenografts. This provides novel methodologic for modelling cascade Moreover, our data indicate that putative bone-metastatic get rapidly lost upon routine cell culture.