Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array.
作者： Yu-Luen Deng , Yi-Je Juang
DOI: 10.1063/1.4793224
关键词: Raman spectroscopy 、 Polystyrene 、 Microfluidics 、 Nanotechnology 、 Sample collection 、 Electric field 、 Raman scattering 、 Materials science 、 Electrokinetic phenomena 、 Biomolecule
摘要: In this study, microneedles which possess sharp tips were utilized to trap and detect the biomolecules. Owing large curvature, of created a substantially high gradient electric field under non-uniform served as not only trapping sites but also substrate for surface enhanced Raman scattering (SERS). Separation polystyrene microparticles with different sizes two kinds biomolecules (Staphylococcus aureus (S. aureus) red blood cells (RBCs)) demonstrated. Moreover, in situ detection S. was performed immediately after separation completed. The results showed that, 15 s sample collection, signals detected greatly through SERS effect.
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