Similar GABAergic inputs in dentate granule cells born during embryonic and adult neurogenesis

摘要: Neurogenesis in the dentate gyrus of hippocampus follows a unique temporal pattern that begins during embryonic development, peaks early postnatal stages and persists through adult life. We have recently shown granule cells born mice acquire remarkably similar afferent connectivity firing behavior, suggesting they constitute homogeneous functional population [Laplagne et al. (2006)PLoS Biol., 4, e409]. Here we extend our previous study by comparing mature neurons hippocampus, with focus on intrinsic membrane properties gamma-aminobutyric acid (GABA)ergic synaptic inputs. For this purpose, dividing neuroblasts ventricular wall were retrovirally labeled green fluorescent protein at day 15 (E15), progenitor subgranular zone red same 42 (P42, adulthood). Electrophysiological either stage then compared brain slices. Evoked spontaneous GABAergic postsynaptic responses perisomatic dendritic origin displayed characteristics both neuronal populations. Miniature inputs also showed pharmacological profile. A comparative analysis present data observations rendered no significant differences among recorded from embryonic, mice. Yet, embryo-born reduced excitability, lower engagement network activity. Our results demonstrate different age, location degree excitability receive equivalent characteristics.