The effects of abnormalities of glucose homeostasis on the expression and binding of muscarinic receptors in cerebral cortex of rats

摘要: Abstract Glucose homeostasis in humans is an important factor for the functioning of nervous system. Both hypo and hyperglycemia contributes to neuronal functional deficit. In present study, effect insulin induced hypoglycemia streptozotocin diabetes on muscarinic receptor binding, cholinergic enzymes; AChE, ChAT expression GLUT3 cerebral cortex experimental rats were analysed. Total muscarinic, M 1 showed a significant decrease 3 subtype increased binding hypoglycemic compared diabetic control. Real-Time PCR analysis , subtypes confirmed studies. Immunohistochemistry receptors using specific antibodies also carried out. AChE up regulated down control rats. Our results that hypo/hyperglycemia caused impaired glucose transport cells as shown by altered GLUT3. Increased decreased suggested alter cortical acetylcholine metabolism along with hypo/hyperglycemic rats, impair transmission, which subsequently lead dysfunction thereby causing learning memory deficits. We observed prominent disturbance condition than hyperglycemia. Hypoglycemia exacerbated neurochemical changes These findings have implications both therapy identification causes contributing diabetes.