Sizes, locations, and directions of transcription of two genes on a cloned maize chloroplast DNA sequence

摘要: Abstract mRNA for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate (dimerizing), EC 4.1.1.39] Zea mays is complementary to an uninterrupted 1600-base-pair-long chloroplast DNA sequence that has been mapped precisely within 4350-base-pair-long fragment Bam 9 which it had traced earlier [Bedbrook, J. R., Coen, D. M., Beaton, A. Bogorad, L. & Rich, (1979) Biol. Chem. 254, 905-910]. An additional 1400-base-pair-long region colinear with a RNA detected on 9. The transcript from this part 2200-nucleotide-long RNA. remainder 2200-base-pair maps outside 1600-base-pair LS gene and 2200-nucleotide are close one another. They separated by untranscribed intercistronic “gap” about 330 base pairs long. These two closely packed genes inverted chromosome—i.e., their 3′ termini at opposite ends gap they map strands.