The effect of nitrogen limitation on acetyl-CoA carboxylase expression and fatty acid content in Chromera velia and Isochrysis aff. galbana (TISO)

摘要: Lipids from microalgae have become a valuable product with applications ranging biofuels to human nutrition. While changes in fatty acid (FA) content and composition under nitrogen limitation are well documented, the involved molecular mechanisms poorly understood. Acetyl-CoA carboxylase (ACCase) is key enzyme FA synthesis elongation pathway. Plastidial cytosolic ACCases provide malonyl-CoA for de novo plastid endoplasmic reticulum, respectively. The present study aimed at investigating expression of plastidial ACCase Chromera velia Isochrysis aff. galbana (TISO) their impact on level when grown nitrogen-deplete conditions. In C. velia, was significantly upregulated during starvation culture age, strongly correlating increased content. Conversely, I. not differentially expressed cultures, but logarithmic phase nitrogen-replete cultures. contrast ACCase, downregulated age nitrogen-starvation, an increase medium-chain FAs. conclusion, changed growth nutrient status species-specific manner did always result accumulation.