Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea

摘要: Abstract Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete N-reduced conditions to determine the effects of N supply medium growth phase on microalgal physiology biochemistry. These two T. compared terms growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, biochemical composition, with a focus class fatty acid compositions. The standard strain ( T ) reached higher cell counts regardless medium. In both strains, final density was significantly lower Carbohydrates enhanced at stationary supply. oleaginous T + accumulated triacylglycerols; whereas, alkenones as reserve lipids, especially phase. Each exhibited an intrinsic specific FA profile neutral lipids (NL) and, lesser extent, polar (PL) independent contained more saturated monounsaturated acids (especially 14:0 18:1n-9), but less polyunsaturated (18:4n-3 22:6n-3) than . Overall, induced changes profiles NL PL