Incorporation of exudates of human platelet‐rich fibrin gel in biodegradable fibrin scaffolds for tissue engineering of cartilage
作者： Chi-Sheng Chien , Hsiu-O Ho , Yu-Chih Liang , Pai-Hung Ko , Ming-Thau Sheu
DOI: 10.1002/JBM.B.32657
关键词: Cartilage metabolism 、 Chondrocyte 、 Tissue engineering 、 Fibrin 、 Thrombin 、 Matrix (biology) 、 Cell biology 、 Cartilage 、 Materials science 、 Regeneration (biology) 、 Biomedical engineering
摘要: The goal of this study was to assess the incorporation exudates human platelet-rich fibrin (hPRF) that is abundant in platelet cytokines and growth factors into biodegradable (FB) scaffolds as a regeneration matrix for promoting chondrocyte proliferation re-differentiation. hPRF obtained from blood by centrifugation without an anticoagulant, exudate collected mixed with bovine fibrinogen, then thrombin added form FB scaffold. Proliferation differentiation primary chondrocytes chondrosarcoma cell line, SW-1353, embedded three-dimensional (3D) on two-dimensional (2D) surface so produced were evaluated comparison agarose (AG) scaffold serving control. Results demonstrated amounts these higher than those blood-derived products except TGF-β1. Chondrocytes SW1353 cells 2D 3D addition PRF exhibited more-available AG scaffolds. It concluded can provide appropriate environment re-differentiation, it could be improved adding hPRF. These have great promise cartilage tissue engineering.
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