TLC Densitometric Method for the Quantification of Eugenol and Gallic Acid in Clove

作者: S. B. Pathak , K. Niranjan , H. Padh , M. Rajani

DOI: 10.1365/S10337-004-0373-Y

关键词: ChromatographyRepeatabilitySyzygiumGallic acidChemistryEugenol

摘要: Eugenol and gallic acid are reported from the flower buds of Syzygium aromaticum (L.) Merr. & Perry (clove). Both compounds have been shown to give interesting biological activities hence serve as biomarkers. We report a simple TLC densitometric method for quantification eugenol in clove. The was validated precision, repeatability accuracy. found be precise with RSD 0.61 1.3 (intraday) 0.96 0.24 (interday) different concentrations respectively. Instrumental precision 0.21 (% CV) Accuracy checked by conducting recovery study at two levels average percentage recoveries were 99.79% 97.90% contents samples clove, estimated proposed method, range 12.9–14.6% 0.31–0.61% HPTLC estimation simple, precise, specific, sensitive accurate can used routine quality control

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