ICSI, Male Pronuclear Remodeling and Cell Cycle Checkpoints

摘要: Intracytoplasmic sperm injection (ICSI; Palermo et al., 1992; Van Steirteghem 1993) has heralded an era of enormous improvements in treating male infertility; it resulted the births thousands babies. In fact its success led some clinicians to claim that infertility is now “cured”, while others raise concerns over global application ICSI for all cases vitro fertilization (Tucker 1995). These may be, part, based on lack supporting pre-clinical studies relevant animal models (Ng 1995; Yanagimachi al, Others are worried about possible long-term effects offspring (Hamberger 1998]; Moutel 1999). While current view one cautious optimism, development a preclinical, non-human primate model been established address safety and other methods assisted reproduction (Hewitson 1998; Hewitson 1999; 2001). Using advanced fluorescent imaging techniques, cytoskeletal events occur during rhesus have examined after both (IVF) ICSI. It was found monkeys share many similarities with humans terms chromatin dynamics by IVF (Simerly Wu 1996). However, oocytes fertilized abnormal nuclear remodeling leading asynchronous decondensation apical region head, delaying onset DNA synthesis. The persistence acrosome perinuclear theca apex introduced into oocyte constrict this Ramalho-Santos 2000a). Despite these differentces, normal embryos produced and, transfer surrogate females, several 2001)