Characterization of human blood dendritic cell subsets.

摘要: Dendritic cells (DCs) are key antigen-presenting for stimulating immune responses and they now being investigated in clinical settings. Although defined as lineage-negative (Lin(-)) HLA-DR(+) cells, significant heterogeneity these preparations is apparent, particularly regard to the inclusion or exclusion of CD14(+), CD16(+), CD2(+) cells. This study used flow cytometry a panel monoclonal antibodies (mAbs), including reagents from 7th Leukocyte Differentiation Antigen Workshop, define cellular composition 2 standardized peripheral blood mononuclear cell (PBMCs)-derived Lin(-) preparations. were prepared PBMCs by depletion with CD3, CD14, CD19, CD11b, either CD16 CD56 mAbs. Analysis CD16-replete divided population into 5 nonoverlapping subsets (mean +/- 1 SD): CD123 = 18.3% 9.7%), CD1b/c (18.6% 7.6%), (49.6% 8.5%), BDCA-3 (2.7% 1.4%), CD34 (5.0% 2.4%). The had distinct phenotypes when compared each other, monocytes, monocyte-derived DCs (MoDCs). CD85 family, C-type lectins, costimulatory molecules, differentiation/activation molecules also expressed differentially on subsets, MoDCs. poor viability CD123(+) vitro was confirmed, but CD16(+) CD11c(+) DC subset survived poorly. Finally, individual stimulators allogeneic mixed leukocyte reactions ranked their allostimulatory capacity > CD34. These data provide an opportunity standardize populations future molecular, functional possibly even therapeutic studies.