ABI5 Interacts with Abscisic Acid Signaling Effectors in Rice Protoplasts

摘要: Abscisic acid (ABA) regulates seed maturation, germination, and adaptation of vegetative tissues to environmental stresses. The mechanisms ABA action the specificity conferred by signaling components in overlapping pathways are not completely understood. ABI5 gene (ABA insensitive 5) Arabidopsis encodes a basic leucine zipper factor required for response tissues. Using transient expression rice protoplasts, we provide evidence functional interactions with effectors VP1 (viviparous 1) ABI1 1). Co-transformation experiments cDNA constructs resulted specific transactivation ABA-inducible wheat Em, AtEm6, bean beta-Phaseolin, barley HVA1 HVA22 promoters. Furthermore, interacted synergistically co-expressed VP1, indicating that is involved ABA-regulated transcription mediated VP1. ABI5-mediated was inhibited overexpression abi1-1, dominant-negative allele protein phosphatase ABI1, 1-butanol, competitive inhibitor phospholipase D signaling. Lanthanum, trivalent ion acts as an agonist signaling, potentiated transactivation. These results demonstrate key target conserved pathway plants.