Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination
作者： Laura Mureşan , Ruben Ronda Valera , Ivo Frébort , Ionel Catalin Popescu , Elisabeth Csöregi
DOI: 10.1007/S00604-008-0033-2
关键词: Biosensor 、 Tyramine 、 Biogenic amine 、 Spermidine 、 Amperometry 、 Chromatography 、 Putrescine 、 Amine oxidase 、 Cadaverine 、 Chemistry
摘要: A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly electrochemical biosensor to weak acid cation-exchange column online detection of amines. bienzyme design, based on recently isolated amine oxidase from grass pea commercial horseradish peroxidase, was used the construction. enzymes were co-immobilized surface graphite electrode together with mediator (Os-redox polymer). performed at low applied potential (-50 mV vs. Ag/AgCl, KCl0.1 M), where biases interferences are minimal. separation determination six BA, relevance food analysis (tyramine, putrescine, cadaverine, histamine, agmatine spermidine), investigated. Irrespective BA nature, oxidase-based showed linear response up 5 mM, its sensitivity decreases following order: spermidine, agmatine, histamine tyramine. approach estimate content fish samples, after their extraction methanesulfonic acid. (Less)
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