A unified method for the assay of uridine diphosphoglucuronyl-transferase activities toward various aglycones using uridine diphospho[U-14C]glucuronic acid
作者： Surendra K. Bansal , T. Gessner
DOI: 10.1016/0003-2697(80)90655-7
关键词: Uridine 、 Glucuronide 、 Microsome 、 Transferase 、 Hydrolysis 、 Glucuronic acid 、 Chemistry 、 Glucuronates 、 Uridine diphosphate glucuronic acid 、 Chromatography 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Abstract A method is described for the assay of UDP-glucuronyltransferase activity various aglycones using UDP-[U 14 -C]glucuronic acid. The radioactive glucuronide conjugates formed during reactions were separated from unmetabolized UDP-[U- C]glucuronic acid, and other uncharacterized products, by thin-layer chromatography. identified comparison their R f values on chromatography with those several authentic glucuronides, hydrolysis β-glucuronidase in absence or presence d -saccharic acid-1,4-lactone, an inhibitor β-glucuronidase. procedure general determination enzymic aglycones. It has been utilized eleven capable extension to many others.
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