Pulsed-field gel electrophoresis-fingerprinting, genome size estimation and rrn loci number of Rhizobium galegae
作者： Ingrid Huber , Sonja Selenska-Pobell
DOI: 10.1111/J.1365-2672.1994.TB04397.X
关键词: Rhizobium galegae 、 Genome size 、 Pulsed-field gel electrophoresis 、 Gel electrophoresis 、 Ribosomal RNA 、 Ribosomal DNA 、 Molecular biology 、 Genetics 、 Restriction enzyme 、 Restriction fragment length polymorphism 、 Biology
摘要: I. HUBER AND S. SELENSKA-POBELL. 1994. The genomes of several Rhizobium galegae (of.) strains, which effectively nodulate Galega officinalis host, were analysed by pulsed-field gel electrophoresis (PFGE). Individual PFGE-fingerprints obtained for every particular strain when the rarely cutting restriction endonucleases SpeI and AsnI applied. In hybridization experiments, where a DNA fragment carrying rrnB ribosomal RNA operon Escherichia coli was used as probe, number resulting strain-specific bands reduced to three all strains studied. This suggests that in Rh. there are at least rrn loci. On basis lengths fragments, separated PFGE, genome size five estimated be 5852 ± 198 kbp.
sci-hub.se 参考文章(17)
Z. Bánfalvi, V. Sakanyan, C. Koncz, A. Kiss, I. Dusha, A. Kondorosi, Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti Molecular and General Genetics MGG. ,vol. 184, pp. 318- 325 ,(1981) , 10.1007/BF00272925
W. J. Kelly, C. M. Huang, R. V. Asmundson, Comparison of Leuconostoc oenos Strains by Pulsed-Field Gel Electrophoresis Applied and Environmental Microbiology. ,vol. 59, pp. 3969- 3972 ,(1993) , 10.1128/AEM.59.11.3969-3972.1993
B W Sobral, R J Honeycutt, A G Atherly, The genomes of the family Rhizobiaceae: size, stability, and rarely cutting restriction endonucleases. Journal of Bacteriology. ,vol. 173, pp. 704- 709 ,(1991) , 10.1128/JB.173.2.704-709.1991
K M Borges, P L Bergquist, Genomic restriction map of the extremely thermophilic bacterium Thermus thermophilus HB8. Journal of Bacteriology. ,vol. 175, pp. 103- 110 ,(1993) , 10.1128/JB.175.1.103-110.1993
Nathalie Bourget, Jean-Marc Simonet, Bernard Decaris, Analysis of the genome of the five Bifidobacterium breve strains: plasmid content, pulsed-field gel electrophoresis genome size estimation and rrn loci number Fems Microbiology Letters. ,vol. 110, pp. 11- 20 ,(1993) , 10.1111/J.1574-6968.1993.TB06288.X
K. LINDSTROM, Rhizobium galegae, a new species of legume root nodule bacteria International Journal of Systematic and Evolutionary Microbiology. ,vol. 39, pp. 365- 367 ,(1989) , 10.1099/00207713-39-3-365
Sonja Selenska-Trajkova, Galina Radeva, Liliana Gigova, Kaltcho Markov, Localization of nif genes on large plasmids in Rhizobium galegae. Letters in Applied Microbiology. ,vol. 11, pp. 73- 76 ,(1990) , 10.1111/J.1472-765X.1990.TB01278.X
Isabel Walls, J. J. Sheridean, R. W. Welch, D. A. McDowell, Separation of micro-organisms from meat and their rapid enumeration using a membrane filtration-epifluorescent microscopy technique Letters in Applied Microbiology. ,vol. 10, pp. 23- 26 ,(1990) , 10.1111/J.1472-765X.1990.TB00086.X
Michael McClelland, Robert Jones, Yogesh Patel, Michael Nelson, Restriction endonucleases for pulsed field mapping of bacterial genomes Nucleic Acids Research. ,vol. 15, pp. 5985- 6005 ,(1987) , 10.1093/NAR/15.15.5985
Viviana Corich, Alessio Giacomini, Francisco J. Ollero, Andrea Squartini, Marco F. Nuti, Pulsed-field electrophoresis in contour-clamped homogeneous electric fields (CHEF) for the fingerprinting of Rhizobium spp. Fems Microbiology Letters. ,vol. 83, pp. 193- 197 ,(1991) , 10.1111/J.1574-6968.1991.TB04439.X-I1