T-h-2 immunity and CD3+CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope.
作者： Vivekanandhan Aravindhan , Sujatha Narayanan , Namasivayam Gautham , Vinayaka Prasad , P. Kannan
DOI: 10.1111/J.1574-695X.2006.00061.X
关键词: Immunoglobulin G 、 T lymphocyte 、 Epitope 、 Antigen 、 Recombinant DNA 、 Virology 、 Antibody 、 Lymphocyte proliferation 、 CD3 、 Microbiology 、 Biology
摘要: The genetic engineering of Mycobacterium bovis-bacillus Calmette-Guerin to express foreign epitopes is an attractive strategy in the field epitope vaccines. We constructed 'epitope-trap vector' with tuberculosis chaperonin-10 as a carrier antigen and used it HIV-1 principal neutralizing determinant epitope. also identified new promoter that was hyperexpressive compared heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette-Guerin-immunized mice showed enhanced lymphocyte proliferation interleukin-4 (but not interferon-gamma) secretion. group exhibited mild delayed-type hypersensitivity reaction high frequency CD3+CD45RBlow-activated T cells, together titer antiprincipal immunoglobulin G antibodies. Thus, this delivery system induced strong epitope-specific T-h-2 polarization.
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