Reduction of early B lymphocyte precursors in transgenic mice overexpressing the murine heat-stable antigen.

摘要: To study the role of murine heat-stable Ag (HSA) in lymphocyte maturation, we generated transgenic mice which HSA cDNA was under transcriptional control TCR V beta promoter and Ig mu enhancer. The transgene expressed during all stages B maturation. Expression first detected earliest lymphoid-committed progenitors, normally do not express HSA, subsequently reached highest levels pro- pre-B cells. In bone marrow, number IL-7-responsive clonogenic progenitors < 4% normal, whereas frequency earlier lymphocyte-restricted precursors, detectable as Whitlock-Witte culture-initiating cells, normal. Pro- cells by flow cytometry were reduced approximately 50% relative to controls. Mature splenic also but a lesser extent than their response LPS stimulation impaired. Reconstitution SCID BALB/c-nu/nu with marrow indicated that perturbations lymphopoiesis caused defective microenvironment or abnormal T Our previous studies showed elevated expression throughout thymocyte development, resulted profound depletion CD4+CD8+ double-positive single-positive thymocytes. Together, these results indicate can determine capacity early lymphoid proliferate survive. Therefore, could serve an important regulator lymphopoiesis.