Estradiol enhances leukocyte binding to tumor necrosis factor (TNF)-stimulated endothelial cells via an increase in TNF-induced adhesion molecules E-selectin, intercellular adhesion molecule type 1, and vascular cell adhesion molecule type 1.

摘要: Adhesion of leukocytes to endothelial cells is a critical step in the development acute and chronic inflammatory lesions. We report here that estradiol treatment cultured human umbilical vein stimulated up twofold increase TNF-induced adhesion both polymorphonuclear PMA-activated peripheral blood mononuclear cells. This effect was more evident (threefold increase) when were on basement membrane glycoprotein laminin. Progesterone, but not testosterone, had similar stimulatory effect. Estradiol also promoted slight interferon gamma-stimulated cell adherence for cells, no observed with IL-1 or IL-4. The estradiol-induced leukocyte binding partially blocked by antibodies molecules E-selectin, intercellular molecule type 1 (ICAM-1), vascular (VCAM-1). Indirect immunofluorescence techniques showed produces an surface expression these molecules. Northern blot analysis demonstrated transient mRNA ICAM-1, VCAM-1 treated estradiol. Our data demonstrate has important regulatory functions promoting leukocyte-endothelial interactions might contribute predominance females some autoimmune diseases.