Effects of Nonoxinol-9 on the Ability to Obtain DNA Profiles from Postcoital Vaginal Swabs

摘要: JFSCA, Vol. 38, No. 2, March 1993, pp. 442-447. ABSTRACT: Nonoxinol-9, the active ingredient of many spermicide foams and creams, has been shown to inactivate effectively high titres HIV in vitro. Therefore early admin- istration nonoxinol-9, perhaps by a rape victim herself, suggested as potential prophylactic therapy for prevention possible infection. For forensic DNA identity testing, it becomes pertinent determine whether nonoxinol-9 could have an adverse effect on recovery molecular weight from postcoital vaginal swabs thereby impact restriction fragment length polymorphism (RFLP) analysis. If mo- lecular can not be recovered, may still proceed with analyses using PCR-based tests. In order investigate effects inserts, gels, or sponges containing were applied either 15 rain pre- 60 post coitus. Postcoital taken one six h after sexual intercourse, was isolated typing performed. The results demonstrate that no negative ability obtain profiles, RFLP PCR-based, swabs. quantity extractable obtained (as determined slot-blot analysis) comparable uncontaminated patterns at HLA-DQ alpha DIS80 loci treated consistent control corresponding whole blood samples donors. administration topical anti-HIV agent is impedient obtaining profiles evidentiary material. KEYWORDS: toxicology, pathology biology, AIDS, HIV, deoxyribonucleic acid (DNA), swabs, identification semen, RFLP, PCR Approximately 2.5 million women United States more throughout world use spermicides form jellies, foams, names commercial manufacturers are provided only inclusion does imply endorsement Federal Bureau Investigation. Received publication 30 1992, revised manuscript received 29 June 1992; accepted 3 July 1992. ~Forensic scientist, research assistant, director respectively, Department Forensic Medicine, University Bern, Switzerland. 2Research chemist, Science Research Training Center, Laboratory Division, FBI Academy, Quantico, VA.