Separation of Listeria monocytogenes and Salmonella berta from a complex food matrix by aqueous polymer two‐phase partitioning

作者: Pedersen , Skouboe , Rossen , Rasmussen

DOI: 10.1046/J.1472-765X.1998.00275.X

关键词: BacteriaListeria monocytogenesStarchPolyethylene glycolAqueous solutionDextranPhase (matter)BiochemistryPolymerChromatographyChemistry

摘要: In the present study, use of aqueous polymer two-phase systems for separation pathogenic bacteria from a complex food sample was investigated. Three different systems, polyethylene glycol 3350/dextran T 500, methoxy 5000/dextran 500 and 3350/hydroxypropyl starch system, were compared at pH 3 6 their capacity to separate Listeria monocytogenes Salmonella berta Cumberland sausage. all three phase particles partitioned lower phase. Best performance obtained by combinations, starch. these hydrophobic upper both with an average partitioning ratio 80% recovery 56%. only 72% 45%. This method may become valuable tool matrices.

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