A conceptual model linking functional gene expression and reductive dechlorination rates of chlorinated ethenes in clay rich groundwater sediment.

作者: Jacob Bælum , Julie C. Chambon , Charlotte Scheutz , Philip J. Binning , Troels Laier

DOI: 10.1016/J.WATRES.2013.02.016

关键词: Environmental chemistryDehalogenaseBacterial growthVinyl chlorideBiologyReductive dechlorinationSteady state (chemistry)DehalococcoidesSedimentEnvironmental engineeringMicrocosm

摘要: We used current knowledge of cellular processes involved in reductive dechlorination to develop a conceptual model describe the regulatory system at cell level; links bacterial growth and substrate consumption abundance messenger RNA functional genes process. The applicability was tested on treatability study biostimulated bioaugmented microcosms. Using quantitative real time PCR, high-resolution expression profiles dehalogenase bvcA vcrA were obtained during two consecutive events trichlorethene, cis-dichlorethene vinyl chloride. Up-regulation (for microcosms) gene fitted well with high rates chloride, while no known transcripts could be measured trichloroethene dechlorination. Maximum concentrations 2.1 1.7 per genes, respectively, same points as maximum observed. developed compared experimental data for both microcosms under non-steady state conditions supported by results from recently published steady conditions.

参考文章(57)
Rosa Krajmalnik-Brown, Youlboong Sung, Kirsti M. Ritalahti, F. Michael Saunders, Frank E. Löffler, Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides FEMS Microbiology Ecology. ,vol. 59, pp. 206- 214 ,(2007) , 10.1111/J.1574-6941.2006.00243.X
Marcio L Da Silva, PJ Alvarez, None, Exploring the Correlation between Halorespirer Biomarker Concentrations and TCE Dechlorination Rates Journal of Environmental Engineering. ,vol. 134, pp. 895- 901 ,(2008) , 10.1061/(ASCE)0733-9372(2008)134:11(895)
Charlotte Scheutz, Neal d. Durant, Philip Dennis, Maria Heisterberg Hansen, Torben Jørgensen, Rasmus Jakobsen, Evan e. Cox, Poul L. Bjerg, Concurrent ethene generation and growth of Dehalococcoides containing vinyl chloride reductive dehalogenase genes during an enhanced reductive dechlorination field demonstration. Environmental Science & Technology. ,vol. 42, pp. 9302- 9309 ,(2008) , 10.1021/ES800764T
Melanie Duhamel, Stephan D. Wehr, Lawrence Yu, Homa Rizvi, David Seepersad, Sandra Dworatzek, Evan E. Cox, Elizabeth A. Edwards, Comparison of anaerobic dechlorinating enrichment cultures maintained on tetrachloroethene, trichloroethene, cis-dichloroethene and vinyl chloride Water Research. ,vol. 36, pp. 4193- 4202 ,(2002) , 10.1016/S0043-1354(02)00151-3
Cong Lu, Poul L. Bjerg, Fengjun Zhang, Mette M. Broholm, Sorption of chlorinated solvents and degradation products on natural clayey tills Chemosphere. ,vol. 83, pp. 1467- 1474 ,(2011) , 10.1016/J.CHEMOSPHERE.2011.03.007
Jacob Bælum, Charlotte Scheutz, Julie C. Chambon, Christine Mosegaard Jensen, Rikke P. Brochmann, Philip Dennis, Troels Laier, Mette M. Broholm, Poul L. Bjerg, Philip J. Binning, Carsten S. Jacobsen, The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till Environmental Pollution. ,vol. 186, pp. 149- 157 ,(2014) , 10.1016/J.ENVPOL.2013.11.013
M. Ahsanul Islam, Elizabeth A. Edwards, Radhakrishnan Mahadevan, Characterizing the Metabolism of Dehalococcoides with a Constraint-Based Model PLoS Computational Biology. ,vol. 6, pp. e1000887- 1680 ,(2010) , 10.1371/JOURNAL.PCBI.1000887
Ida Damgaard, Poul L. Bjerg, Jacob Bælum, Charlotte Scheutz, Daniel Hunkeler, Carsten S. Jacobsen, Nina Tuxen, Mette M. Broholm, Identification of chlorinated solvents degradation zones in clay till by high resolution chemical, microbial and compound specific isotope analysis Journal of Contaminant Hydrology. ,vol. 146, pp. 37- 50 ,(2013) , 10.1016/J.JCONHYD.2012.11.010
Donna E. Fennell, James M. Gossett, Modeling the Production of and Competition for Hydrogen in a Dechlorinating Culture Environmental Science & Technology. ,vol. 32, pp. 2450- 2460 ,(1998) , 10.1021/ES980136L