Peripheral blood based C-PCR assay for diagnosing extra-pulmonary tuberculosis.

摘要: Extra pulmonary tuberculosis (EPTB) constitutes around 20% of all cases in India. Conventional methods are limited use diagnosing this form the disease. Polymerase chain reaction (PCR) has emerged as a sensitive and specific tool for documenting presence Mycobacterium clinical samples but lacks quantitative ability. The present study evaluates peripheral blood an alternative specimen EPTB. Peripheral from 38 EPTB 89 non tuberculous subjects were analyzed tubercle bacilli by MPB 64 gene based PCR method. assay gave overall sensitivity 60.53% with negative predictive value 76.92% which is superior to gold standard mycobacterial culture (10.53 72.36%). Additionally, 43.82% positive results PCR, thus mitigating utility test. An in-house Competitive (C-PCR) was used determine load positive, patients controls ranged 7498 – 12498, 602 4797 101 800 genome equivalent (ge)/mL, respectively. data clearly demonstrated that C-PCR can furnish insightful information extra