Use of Green Fluorescent Protein-Conjugated β-Actin as a Novel Molecular Marker for in Vitro Tumor Cell Chemotaxis Assay

摘要: To study the dynamics of actin cytoskeleton rearrangement in living cells, an eukaryotic expression vector expressing a β-actin-GFP fusion protein was generated. The construct when transfected into NIH3T3 fibroblast, A2058 human melanoma and 293T embryonic kidney carcinoma cell lines expressed protein, which colocalized with endogenous cellular as determined by histoimmunofluorescence staining. also observed to be reorganized response treatments chemoattractant type IV collagen. Cells extended pseudopodial protrusions altered morphology their cortical structure collagen stimulation. More importantly, accumulated areas undergoing these dynamic changes, indicating that could participate polymerization. Although ectopic lead minor side effects on proliferation, studies suggest this strategy provides alternative invasive techniques currently used permits real-time visualization rearrangements environmental cues.