Deposition of newly synthesized histones: new histones H2A and H2B do not deposit in the same nucleosome with new histones H3 and H4.
作者： Vaughn Jackson
DOI: 10.1021/BI00382A037
关键词: Histone H4 、 Biochemistry 、 Biophysics 、 Histone 、 Gel electrophoresis 、 Histone H2B 、 Chemistry 、 Chromatin 、 Nucleosome 、 Histone H3 、 Histone octamer
摘要: The authors have developed procedures to study histone-histone interactions during the deposition of histones in replicating cells. Cells are labeled for 60 min with dense amino acids, and subsequently, within nucleosomes cross-linked into an octameric complex formaldehyde. These complexes sedimented equilibrium density gradients octamer dioctamer separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. With reversal cross-link, distribution individual density-labeled is determined. Newly synthesized H3 H4 deposits as a tetramer associated old H2A H2B. H2B deposit dimer H2A, H2B, H3, H4. significance these results respect dynamics histone nucleus discussed. Control experiments presented test artifactual formation preparative procedures. In addition, reconstitution were performed demonstrate that composition can be determined from their gradients.
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