Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium

摘要: Compartmentalization of cellular signaling forms the molecular basis behavior. The primary cilium constitutes a subcellular compartment that orchestrates signal transduction independent from cell body. Ciliary dysfunction causes severe diseases, termed ciliopathies. Analyzing ciliary has been challenging due to lack tools investigate signaling. Here, we describe nanobody-based targeting approach for optogenetic in mammalian cells and vivo zebrafish specifically analyze function. Thereby, overcome loss protein function observed after fusion sequences. We functionally localized modifiers cAMP signaling, photo-activated adenylyl cyclase bPAC light-activated phosphodiesterase LAPD, biosensor mlCNBD-FRET cilium. Using this approach, studied contribution spatial controlling cilia length. Combining optogenetics with will pave way understanding health disease.