One-step reduced/quinone functionalized graphene oxide as reagentless lactate biosensing platform

作者: Iria Bravo , Mónica Revenga-Parra , Karina Weber , Jürgen Popp , Félix Pariente

DOI: 10.1016/J.SNB.2018.03.170

关键词: OxideChemistrySchiff baseCombinatorial chemistryHydroquinoneBiosensorElectrochemistryNanomaterialsQuinoneGraphene

摘要: Abstract One-step approach to simultaneously reduce and modify graphene oxide (GO) using N,N’-Bis(3,4-dihydroxybenzylidene)-1,2-diaminobenzene Schiff base ligand (3,4DHS), in order obtain functionalized reduced (rGO-DHS), is described. Once the novel hybrid nanomaterial was characterized by spectroscopic electrochemical techniques, confirming presence of characteristic quinone/hydroquinone moieties, it has been combined with lactate oxidase (LOx) develop a sensitive selective reagentless biosensor. The resulting biosensing device shows very good analytical performance detection determination limits 2.9 9.7 μM, respectively. RSD for three different devices lower than 9%. operational stability more one month. applicability demonstrated direct l -lactate content white wine.

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