Spectral properties of fluorescent flavoproteins of isolated rat liver mitochondria.

作者: Wolfram S. Kunz

DOI: 10.1016/0014-5793(86)80137-5

关键词: FlavoproteinFluorescenceDehydrogenaseRedoxBiochemistryElectron transferChemistryRat liver mitochondriaFlavin groupEmission spectrum

摘要: The different flavoproteins contributing to flavin fluorescence of isolated rat liver mitochondria have distinct excitation and emission spectra. NAD-linked component was identified as α-lipoamide dehydrogenase, while the non-NAD-linked found be electron transfer flavoprotein. differences in properties mitochondrial permit selective recording their redox state changes mitochondria.

参考文章(15)
Paul A. Srere, Ronald W. Estabrook, Microenvironments and metabolic compartmentation Academic Press. ,(1978)
R Scholz, R G Thurman, J R Williamson, B Chance, T Bücher, Flavin and pyridine nucleotide oxidation-reduction changes in perfused rat liver. I. Anoxia and subcellular localization of fluorescent flavoproteins. Journal of Biological Chemistry. ,vol. 244, pp. 2317- 2324 ,(1969) , 10.1016/S0021-9258(19)78228-3
F Itshak, B Chance, R Oshino, B Schoener, Y Nakase, Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. NADH and flavoprotein fluorescence signals. Journal of Biological Chemistry. ,vol. 254, pp. 4764- 4771 ,(1979) , 10.1016/S0021-9258(17)30079-0
A. J. W. G. VISSER, J. S. SANTEMA, A. HOEK, SPECTROSCOPIC AND DYNAMIC CHARACTERIZATION OF FMN IN REVERSED MICELLES ENTRAPPED WATER POOLS Photochemistry and Photobiology. ,vol. 39, pp. 11- 16 ,(1984) , 10.1111/J.1751-1097.1984.TB03397.X
Balázs Sümegi, József Batke, Zoltán Porpáczy, Substrate-induced structural changes of the pyruvate dehydrogenase multienzyme complex Archives of Biochemistry and Biophysics. ,vol. 236, pp. 741- 752 ,(1985) , 10.1016/0003-9861(85)90680-0
E.Matti Nuutinen, J.Kalervo Hiltunen, Ilmo E. Hassinen, THE GLUTAMATE DEHYDROGENASE SYSTEM AND THE REDOX STATE OF MITOCHONDRIAL FREE NICOTINAMIDE ADENINE DINUCLEOTIDE IN MYOCARDIUM FEBS Letters. ,vol. 128, pp. 356- 360 ,(1981) , 10.1016/0014-5793(81)80116-0
Ilmo Hassinen, Britton Chance, Oxidation-reduction properties of the mitochondrial flavoprotein chain Biochemical and Biophysical Research Communications. ,vol. 31, pp. 895- 900 ,(1968) , 10.1016/0006-291X(68)90536-6